![]() Protein Electrophoresis & Western Blotting. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare. Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low-femtogram-level detection by Western blot analysis with horseradish peroxidase (HRP). It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.īecause the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. Pierce ECL Western Blotting.Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 µg/mL)Ĭompare and learn more about our western blot chemiluminescent substrates ›.Compatibility: nitrocellulose and PVDF membranes Compare and learn more about our western blot chemiluminescent substrates View all HRP substrates available Pierce ECL Western Blotting Substrate provides.This reaction produces a prolonged chemiluminescence which can be. Pierce ECL Western Blotting Substrate enables the detection of picogram amounts of antigen. Stability: 1-hr working solution stability, 1-year kit stability at 4☌ Thermo Scientific Pierce ECL Western Blotting Substrate is an, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. ![]() Pierce ECL Plus Western Blotting Substrate characteristics include: When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. SuperSignal West Femto Maximum Sensitivity Substrate is designed for low femtogram-level detection by western blot analysis. Pierce ECL Plus Western Blotting Substrate requires more dilute antibody concentrations than those used with precipitating colorimetric HRP substrates.
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